The role of zinc-bound water in liver alcohol dehydrogenase catalysis.
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منابع مشابه
The Role of Zinc - bound Water in Liver Alcohol Dehydrogenase
To elucidate the role of zinc-bound water in liver alcohol dehydrogenase catalysis, chelation by 1,lO-phenanthroline and 2.2-bipyridine was studied. The rate constants for association of both chelating agents to the active center zinc were pH-dependent with a pK, of 9.2 and preferential binding to a protonated form. The binary complex dissociation rate constants were pHindependent for both chel...
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During catalysis by liver alcohol dehydrogenase (ADH), a water bound to the catalytic zinc is replaced by the oxygen of the substrates. The mechanism might involve a pentacoordinated zinc or a double-displacement reaction with participation by a nearby glutamate residue, as suggested by studies of human ADH3, yeast ADH1, and some other tetrameric ADHs. Zinc coordination and participation of wat...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1980
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)86061-1